Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spleen tissue)
Specification
Spleen tissue
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 15°C
Other product details
Concentration
4 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 15°C · Diluent: PBS with 2% goat serum
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rat IgM
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
4% PFA was used to fix sections for 10min at RT. Incubation with primary worked at RT for 1h or overnight at 4C. Washes were with PBS.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Lynda Coughlan
Verified customer
提交于 Dec 15 2010