重组Alexa Fluor® 647荧光Anti-SGK1抗体[Y238] (ab198331)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [Y238] to SGK1
- Suitable for: ICC/IF
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 647荧光Anti-SGK1抗体[Y238]
参阅全部 SGK1 一抗 -
描述
Alexa Fluor® 647荧光兔单克隆抗体[Y238] to SGK1 -
宿主
Rabbit -
偶联物
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: differentiated Neuro2a cells.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y238 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab198331于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100.
This product gave a positive signal in differentiated Neuro2a cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) |
说明 |
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ICC/IF
1/100. This product gave a positive signal in differentiated Neuro2a cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) |
靶标
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功能
Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1. -
组织特异性
Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. -
结构域
Isoform 2 subcellular localization at the plasma membrane is mediated by the sequences within the first 120 amino acids. -
翻译后修饰
Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1.
Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid. -
细胞定位
Cell membrane and Cytoplasm. Nucleus. Endoplasmic reticulum. Nuclear, upon phosphorylation. - Information by UniProt
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数据库链接
- Entrez Gene: 6446 Human
- Entrez Gene: 20393 Mouse
- Entrez Gene: 29517 Rat
- Omim: 602958 Human
- SwissProt: O00141 Human
- SwissProt: Q9WVC6 Mouse
- SwissProt: Q06226 Rat
- Unigene: 510078 Human
see all -
别名
- OTTHUMP00000017247 antibody
- Serine/threonine protein kinase SGK antibody
- Serine/threonine protein kinase Sgk1 antibody
see all
图片
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ab198331 staining SGK1 in differentiated Neuro2a cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198331 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in differentiated Neuro2a cells fixed with 100% methanol (5 min).
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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