Application
Immunohistochemistry (Frozen sections)
Blocking step
PBS, 1% BSA, 10% goat serum, 0.1% TX-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Tissue sections (E9.5 embryo)
Specification
E9.5 embryo
Permeabilization
Yes - 0.5* Triton X-100
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS, 1% BSA, 10% goat serum, 0.1% TX-100
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 594 goat anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/200
Additional data
Additional Notes
Fixed in 4% PFA prior to cryosectioning
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提交于 Jan 30 2014