Application
Western blot
Sample
Human Cell lysate - whole cell (benign prostate and prostate cancer cell lines)
Loading amount
50 µg
Specification
benign prostate and prostate cancer cell lines
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% Milk in TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Rabbit HRP (Cell Signalling)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
West Pico
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 26 kDa Non-specific: 26 kDa
Positive control
benign prostate cell lines BPH-1 and PWR-1E, and prostate cancer cell lines DU145, PC3, LNCaP and 22Rv1
Additional data
Additional Notes
no band detected at correct size, non specific band at 26kDA detected in 4-5/6 cell lines.
Abcam response
Thank you for your honest and valuable feedback. We are sorry this antibody has not worked under your experimental conditions. Many thanks for your feedback on this antibody, we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Darragh O' Donovan
Verified customer
提交于 Dec 31 2012