Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (3T3)
Specification
3T3
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: PBS/BSA
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/500
Additional data
Additional Notes
The a/b was also tried at the dilutions 1/1000 and 1/500
Abcam response
We would like to thank the reviewer for submitting this Abreview for product ab78298. As this product had not yet been tested in ICC/IF on mouse we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received a free primary antibody of their choice for performing these tests. We wish to thank them for participating in our 100% Abreview testing discount program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Marina Shenkman
Verified customer
提交于 Apr 27 2012