Anti-SEC61B抗体(ab15576)
Key features and details
- Rabbit polyclonal to SEC61B
- Suitable for: ICC/IF
- Reacts with: Mouse, Rat, Dog, Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-SEC61B抗体
参阅全部 SEC61B 一抗 -
描述
兔多克隆抗体to SEC61B -
宿主
Rabbit -
特异性
Ab15576 detects human, dog, pig, and rat sec61 beta from microsomes and shows no cross-reactivity with other sec61 isoforms. -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Dog, Human, Pig -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: MDCK cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab15576于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Necessary for protein translocation in the endoplasmic reticulum. -
序列相似性
Belongs to the SEC61-beta family. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 404018 Dog
- Entrez Gene: 10952 Human
- Entrez Gene: 66212 Mouse
- Entrez Gene: 298068 Rat
- Omim: 609214 Human
- SwissProt: P60467 Dog
- SwissProt: P60468 Human
- SwissProt: Q9CQS8 Mouse
see all -
别名
- Protein translocation complex beta antibody
- Protein transport protein SEC61 beta subunit antibody
- Protein transport protein Sec61 subunit beta antibody
see all
图片
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Immunofluorescence analysis of SEC61B was performed using 70% confluent log phase MDCK cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SEC61B Rabbit Polyclonal Antibody (ab15576) at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
数据表及文件
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Datasheet download
文献 (4)
ab15576 被引用在 4 文献中.
- Xu F et al. COPII mitigates ER stress by promoting formation of ER whorls. Cell Res 31:141-156 (2021). PubMed: 32989223
- Bogdanov V et al. Distributed synthesis of sarcolemmal and sarcoplasmic reticulum membrane proteins in cardiac myocytes. Basic Res Cardiol 116:63 (2021). PubMed: 34713358
- Liu X et al. The BEACH-containing protein WDR81 coordinates p62 and LC3C to promote aggrephagy. J Cell Biol 216:1301-1320 (2017). PubMed: 28404643
- Zhang R et al. A CRISPR screen defines a signal peptide processing pathway required by flaviviruses. Nature 535:164-8 (2016). PubMed: 27383988