Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
Sample
Mouse Tissue sections (adult testes)
Specification
adult testes
Permeabilization
No
Fixative
4% PFA
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: donkey Anti-Rabbit IgG (H+L)711-025-152 Jackson
Host species: Donkey
Clonality: Polyclonal
Conjugation: TRITC
Host species: Donkey
Clonality: Polyclonal
Conjugation: TRITC
Dilution
1/200
Additional data
Abcam response
Thank you for your valuable data. Although ab15093 produces the expected nuclear staining pattern, background levels appear high under these experimental conditions. Abcam recommends optimising antigen retrieval conditions for this antibody. For further information, please contact scientific support.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Qing Wen
Verified customer
提交于 May 29 2013