Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Non-reduced Denaturing (14%)
Sample
Human Cell lysate - whole cell (CLL cells)
Specification
CLL cells
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 4% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 2% milk
Dilution
1/6000
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (whole molecule)–Alkaline Pho
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution
1/2500
Detection
Detection method
NBT/BCIP
Bands
Specific: 19, 32 kDa Non-specific: 118, 70, 10, 17 kDa
Exposure
30 minute(s) and 0 second(s)
Positive control
Recombinant soluble SCF(lab-made). Extracted from bacterial inclusion bodies using 1M L-Arginine.
Additional data
Additional Notes
In the first sample (recombinant soluble SCF), the two bands (predicted and observed, always according to the image on your site) are observed. However in the human samples only the predicted bands are observed (around 31 kDa). I am a little bit buffled from your image so i wanted to ask if it is normal that in my human samples the predicted bands are obtained.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. George Gavriilidis
Verified customer
提交于 Feb 26 2015