Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Hippocampus-Paraffin embedded)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9
Permeabilization
No
Specification
Hippocampus-Paraffin embedded
Blocking step
Scy-tek Superblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: in Scy-tek Superblock
Dilution
1/500
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-mouse-Vector Laboratories
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Shows speckles in nuclei as expected.
Signal development for 5min with DAB kit from Vector Labs.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 16 2016