Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 (kidney cell))
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gradient)
Loading amount
5 µg
Specification
HEK293 (kidney cell)
Blocking step
Licor Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Licor Blocking Reagent/PBS 1:1
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat-anti-Mouse IR Dye 800
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
fluorescence
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 35 kDa Non-specific: n/a kDa
Additional data
Additional Notes
This antibody produces a band at the expected molecular weight of 35 kDa with no non-specific bands observed.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 18 2015