Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step
None
Sample
Rat Tissue sections (E9.5 rat placenta)
Specification
E9.5 rat placenta
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS with 3% goat serum
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 Goat Anti-Rabbit IgG from Molecula
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/2000
Additional data
Additional Notes
SATB2 antibody was counter-stained with nuclear marker DAPI.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 11 2013