重组Anti-SATB1抗体[EPR3951] (ab109122)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3951] to SATB1
- Suitable for: IP, ICC/IF, WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-SATB1抗体[EPR3951]
参阅全部 SATB1 一抗 -
描述
兔单克隆抗体[EPR3951] to SATB1 -
宿主
Rabbit -
经测试应用
适用于: IP, ICC/IF, WB, IHC-P, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HAP1, Jurkat and THP1 whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3951 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109122于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
1/20.
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ICC/IF |
1/50.
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|
WB |
1/1000. Predicted molecular weight: 86 kDa.
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|
IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
Flow Cyt |
1/20.
|
说明 |
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IP
1/20. |
ICC/IF
1/50. |
WB
1/1000. Predicted molecular weight: 86 kDa. |
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
Flow Cyt
1/20. |
靶标
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功能
Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly upregulating metastasis-associated genes while downregulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis. -
组织特异性
Expressed predominantly in thymus. -
序列相似性
Belongs to the CUT homeobox family.
Contains 2 CUT DNA-binding domains.
Contains 1 homeobox DNA-binding domain. -
翻译后修饰
Sumoylated. Sumoylation promotes cleavage by caspases.
Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation.
Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms can not dimerize and lose transcription regulation function because of impaired DNA and chromatin association. -
细胞定位
Nucleus matrix. Nucleus > PML body. Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies. - Information by UniProt
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数据库链接
- Entrez Gene: 6304 Human
- Entrez Gene: 20230 Mouse
- Entrez Gene: 316164 Rat
- Omim: 602075 Human
- SwissProt: Q01826 Human
- SwissProt: Q60611 Mouse
- Unigene: 517717 Human
- Unigene: 311655 Mouse
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形式
There are 2 isoforms produced by alternative splicing. -
别名
- DNA binding protein SATB1 antibody
- DNA-binding protein SATB1 antibody
- SATB homeobox 1 antibody
see all
图片
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All lanes : Anti-SATB1 antibody [EPR3951] (ab109122) at 1/1000 dilution (Purified)
Lane 1 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lane 3 : Mouse liver lysate
Lane 4 : Mouse thymus lysate
Lane 5 : Rat thymus lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 86 kDa -
All lanes : Anti-SATB1 antibody [EPR3951] (ab109122) at 1/1000 dilution (Purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) nuclear extract lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) without nuclear extract lysate
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 86 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling SATB1 with Purified ab109122 at 1:500 dilution (0.246 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling SATB1 with Purified ab109122 at 1:500 dilution (0.246 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling SATB1 with Purified ab109122 at 1:500 dilution (0.246 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Purified ab109122 at 1:20 dilution (0.6µg) immunoprecipitating SATB1 in Rat thymus lysate.
Lane 1 (input): Rat thymus lysate 10 µg
Lane 2 (+): ab109122 + Rat thymus lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109122 in Rat thymus lysate.
VeriBlot for IP Detection Reagent (HRP)(ab131366) (1:5000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: kDa -
Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labelling SATB1 with Purified ab109122 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling SATB1 with Purified ab109122 at 1:50 dilution (2.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: SATB1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jurkat whole cell lysate (20 µg)
Lane 4: THP1 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab109122 observed at 100 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab109122 was shown to specifically react with SATB1 in wild-type HAP1 cells as signal was lost in SATB1 knockout cells. Wild-type and SATB1 knockout samples were subjected to SDS-PAGE. Ab109122 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab109122 被引用在 20 文献中.
- Chaurio RA et al. TGF-β-mediated silencing of genomic organizer SATB1 promotes Tfh cell differentiation and formation of intra-tumoral tertiary lymphoid structures. Immunity 55:115-128.e9 (2022). PubMed: 35021053
- Feng D et al. Chromatin organizer SATB1 controls the cell identity of CD4+ CD8+ double-positive thymocytes by regulating the activity of super-enhancers. Nat Commun 13:5554 (2022). PubMed: 36138028
- Yu W et al. Transcriptional regulation of Satb1 in mouse trophoblast stem cells. Front Cell Dev Biol 10:918235 (2022). PubMed: 36589740
- Wright CM et al. scRNA-Seq Reveals New Enteric Nervous System Roles for GDNF, NRTN, and TBX3. Cell Mol Gastroenterol Hepatol 11:1548-1592.e1 (2021). PubMed: 33444816
- Mao L et al. Combination of oncolytic adenovirus targeting SATB1 and docetaxel for the treatment of castration-resistant prostate cancer. J Cancer 12:1846-1852 (2021). PubMed: 33613773