Application
Western blot
Sample
Human Cell lysate - whole cell (RAJI B-Cells)
Loading amount
20 µg
Specification
RAJI B-Cells
Gel Running Conditions
Reduced Denaturing (12% Tris-HEPES-SDS Gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% Milk Block
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
SuperSignal West Pico Chemiluminescent
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: 80 kDa Non-specific: 78, 34, 20 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 14 2009