HRP Anti-SF3a66抗体[4G8] (ab201747)
Key features and details
- HRP Mouse monoclonal [4G8] to SF3a66
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
HRP Anti-SF3a66抗体[4G8]
参阅全部 SF3a66 一抗 -
描述
HRP小鼠单克隆抗体[4G8] to SF3a66 -
宿主
Mouse -
偶联物
HRP -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rat, Monkey -
免疫原
Full length native protein (purified) corresponding to Rat SF3a66. Nuclear protein isolated from rat liver nuclei.
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表位
C-terminal, amino acids 212-464. -
阳性对照
- IHC-P: FFPE human skin tissue sections.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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纯度
Affinity purified -
克隆
单克隆 -
克隆编号
4G8 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab201747于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. -
序列相似性
Belongs to the SF3A2 family.
Contains 1 matrin-type zinc finger. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8175 Human
- Entrez Gene: 299620 Rat
- Omim: 600796 Human
- SwissProt: Q15428 Human
- SwissProt: Q6AXT8 Rat
- Unigene: 115232 Human
- Unigene: 102569 Rat
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别名
- pre-mRNA splicing factor SF3A, subunit 2 antibody
- PRP11 antibody
- PRPF11 antibody
see all
图片
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IHC image of SF3a66 staining in a section of formalin-fixed paraffin-embedded human normal skin*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab201747, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab201747 尚未被引用在任何文献中。