重组Anti-SAM68抗体[EPR3231] (ab76471)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3231] to SAM68
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SAM68抗体[EPR3231]
参阅全部 SAM68 一抗 -
描述
兔单克隆抗体[EPR3231] to SAM68 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human SAM68 (N terminal). The exact sequence is proprietary.
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阳性对照
- HeLa and Jurkat cell lysates and human brain tissue and Hela cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3231 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76471于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/20000 - 1/100000. Detects a band of approximately 68 kDa (predicted molecular weight: 49 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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说明 |
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WB
1/20000 - 1/100000. Detects a band of approximately 68 kDa (predicted molecular weight: 49 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
靶标
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功能
Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export.
Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase. -
组织特异性
Ubiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver. -
序列相似性
Belongs to the KHDRBS family.
Contains 1 KH domain. -
发展阶段
Isoform 3 is only expressed in growth-arrested cells. -
结构域
The KH domain is required for binding to RNA.
The Pro-rich domains are flanked by Arg/Gly-rich motifs which can be asymmetric dimethylated on arginine residues to give the DMA/Gly-rich regions. Selective methylation on these motifs can modulate protein-protein interactions. -
翻译后修饰
Tyrosine phosphorylated by several non-receptor tyrosine kinases, for example LCK, FYN and JAK3. Negatively correlates with ability to bind RNA but required for many interactions with proteins.
Acetylated. Positively correlates with ability to bind RNA.
Arginine methylation is required for nuclear localization. Also can affect interaction with other proteins. Inhibits interaction with Src-like SH3 domains, but not interaction with WW domains of WBP4/FBP21 AND FNBP4/FBP30.
Arg-291, Arg-331 and Arg-346 are found to be also dimethylated, probably to asymmetric dimethylarginine. -
细胞定位
Nucleus. Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10657 Human
- Entrez Gene: 20218 Mouse
- Entrez Gene: 117268 Rat
- Omim: 602489 Human
- SwissProt: Q07666 Human
- SwissProt: Q3U8T3 Mouse
- SwissProt: Q60749 Mouse
- SwissProt: Q91V33 Rat
see all -
别名
- FLJ34027 antibody
- GAP associated tyrosine phosphoprotein p62 antibody
- GAP-associated tyrosine phosphoprotein p62 antibody
see all
图片
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All lanes : Anti-SAM68 antibody [EPR3231] (ab76471) at 1/100000 dilution
Lane 1 : Hela cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 49 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted? -
ab76471 at 1/100 dilution staining SAM68 in human brain tissue, using a HRP/AP polymerized secondary antibody.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunofluorescent staining of HeLa cells using ab76471 at a 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab76471 被引用在 3 文献中.
- Komiyama T et al. High expression of Sam68 contributes to metastasis by regulating vimentin expression and a motile phenotype in oral squamous cell carcinoma. Oncol Rep 48:N/A (2022). PubMed: 36082807
- Rajanala K & Nandicoori VK Localization of nucleoporin Tpr to the nuclear pore complex is essential for Tpr mediated regulation of the export of unspliced RNA. PLoS One 7:e29921 (2012). WB ; Human . PubMed: 22253824
- Cardnell RJ & Mikkelsen RB Nitric oxide synthase inhibition enhances the antitumor effect of radiation in the treatment of squamous carcinoma xenografts. PLoS One 6:e20147 (2011). IHC . PubMed: 21647438