Anti-SAE1抗体(ab97523)
Key features and details
- Rabbit polyclonal to SAE1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SAE1抗体
参阅全部 SAE1 一抗 -
描述
兔多克隆抗体to SAE1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant fragment, corresponding to a region within amino acids 3-217 of Human SAE1 (AAH00344)
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阳性对照
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cells, A549 Xenograft tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97523于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 38 kDa.
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IHC-P |
1/100 - 1/250.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 38 kDa. |
IHC-P
1/100 - 1/250. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. -
组织特异性
Expression level increases during S phase and drops in G2 phase (at protein level). -
通路
Protein modification; protein sumoylation. -
序列相似性
Belongs to the ubiquitin-activating E1 family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 505512 Cow
- Entrez Gene: 10055 Human
- Entrez Gene: 56459 Mouse
- Entrez Gene: 308384 Rat
- Omim: 613294 Human
- SwissProt: A2VE14 Cow
- SwissProt: Q9UBE0 Human
- SwissProt: Q9R1T2 Mouse
see all -
别名
- Activator of SUMO1 antibody
- AOS1 antibody
- HSPC140 antibody
see all
图片
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All lanes : Anti-SAE1 antibody (ab97523) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 38 kDa
10% SDS-PAGE. -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97523 at 1/200 dilution. Lower image is merged with DNA probe.
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Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using ab97523 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab97523 被引用在 2 文献中.
- Wang Q et al. The Expression and Prognostic Value of SUMO1-Activating Enzyme Subunit 1 and Its Potential Mechanism in Triple-Negative Breast Cancer. Front Cell Dev Biol 9:729211 (2021). PubMed: 34621746
- Lapaquette P et al. Shigella entry unveils a calcium/calpain-dependent mechanism for inhibiting sumoylation. Elife 6:N/A (2017). PubMed: 29231810