Anti-S100A9 + Calprotectin (S100A8/A9 complex)抗体[MAC387] (ab22506)
Key features and details
- Mouse monoclonal [MAC387] to S100A9 + Calprotectin (S100A8/A9 complex)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-S100A9 + Calprotectin (S100A8/A9 complex)抗体[MAC387]
参阅全部 S100A9 + Calprotectin (S100A8/A9 complex) 一抗 -
描述
小鼠单克隆抗体[MAC387] to S100A9 + Calprotectin (S100A8/A9 complex) -
宿主
Mouse -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Monkey, Baboon不与反应: Rat -
免疫原
Tissue, cells or virus corresponding to Human S100A9 + Calprotectin (S100A8/A9 complex). Affinity purified solubilized human monocyte preparation.
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阳性对照
- IHC-P: Human Hodgkin's lymphoma tissue.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
MAC387 -
骨髓瘤
NS1 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab22506于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (18) |
Use a concentration of 1 µg/ml.
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说明 |
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IHC-P
Use a concentration of 1 µg/ml. |
图片
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ab22506 staining Macrophages in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with 0.250% casein for 5 minutes at 25°C; antigen retrieval was by proteinase K. Samples were incubated with primary antibody (1/5000 in Tris-HCL) for 30 minutes at 25°C. A HRP-conjugated labeled polymer was used as the secondary antibody.
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IHC image of Macrophage staining in human Hodgkins lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
数据表及文件
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SDS download
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Datasheet download
文献 (82)
ab22506 被引用在 82 文献中.
- Sinha S et al. Dexamethasone modulates immature neutrophils and interferon programming in severe COVID-19. Nat Med 28:201-211 (2022). PubMed: 34782790
- Khamehgir-Silz P et al. Comparative lipid profiling of murine and human atherosclerotic plaques using high-resolution MALDI MSI. Pflugers Arch 474:231-242 (2022). PubMed: 34797426
- Sinha S et al. Fibroblast inflammatory priming determines regenerative versus fibrotic skin repair in reindeer. Cell 185:4717-4736.e25 (2022). PubMed: 36493752
- Sparks HD et al. Biomechanics of Wound Healing in an Equine Limb Model: Effect of Location and Treatment with a Peptide-Modified Collagen-Chitosan Hydrogel. ACS Biomater Sci Eng 7:265-278 (2021). PubMed: 33342210
- Schirmer L et al. Chemokine-Capturing Wound Contact Layer Rescues Dermal Healing. Adv Sci (Weinh) 8:e2100293 (2021). PubMed: 34278740