重组Anti-S100A10抗体[EPR3317] - BSA and Azide free (ab232524)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3317] to S100A10 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-S100A10抗体[EPR3317] - BSA and Azide free
参阅全部 S100A10 一抗 -
描述
兔单克隆抗体[EPR3317] to S100A10 - BSA and Azide free -
宿主
Rabbit -
特异性
The mouse recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse samples.
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经测试应用
适用于: IHC-P, WBmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HaCaT, A431, SW480, RAW264.7, NIH/3T3, and HT-29 cell lysates; IHC-P: Human squamous cell cervical carcinoma, colon tissue and human kidney tissue.
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常规说明
ab232524 is the carrier-free version of ab76472.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3317 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232524于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
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说明 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa. |
靶标
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功能
Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase. -
序列相似性
Belongs to the S-100 family. - Information by UniProt
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数据库链接
- Entrez Gene: 6281 Human
- Entrez Gene: 20194 Mouse
- Omim: 114085 Human
- SwissProt: P60903 Human
- SwissProt: P08207 Mouse
- Unigene: 143873 Human
- Unigene: 1 Mouse
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别名
- 42C antibody
- AA409961 antibody
- AL024248 antibody
see all
图片
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This data was developed using ab76472, the same antibody clone in a different buffer formulation.
Immunohistochemical staining of paraffin embedded human squamous cell cervical carcinoma with purified ab76472 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/10000 dilution (unpurified)
Lane 1 : HaCaT (Human skin keratinocyte) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 3 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa (possible non-specific binding)
Exposure time: 180 secondsBlocking buffer: 5% NFDM/TBST
This data was developed using ab76472, the same antibody clone in a different buffer formulation.
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Lane 1 : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/1000 dilution (purified)
Lane 2 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/5000 dilution
All lanes :Recombinant Human S100A10 protein (His tag) (ab221399)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa (possible dimer)
Exposure time: 3 secondsBlocking buffer: 5% NFDM/TBST
This data was developed using ab76472, the same antibody clone in a different buffer formulation.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
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