Anti-Rif1抗体(ab70254)
Key features and details
- Rabbit polyclonal to Rif1
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Rif1抗体
参阅全部 Rif1 一抗 -
描述
兔多克隆抗体to Rif1 -
宿主
Rabbit -
特异性
Reactivity with mouse Rif1 is not expected owing to divergence of sequence between this ortholog and human Rif1 at the site to which the epitope maps. -
经测试应用
适用于: IHC-P, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Orangutan -
免疫原
Synthetic peptide corresponding to a region between residues 1 and 50 of human Rif1 (NP_060621.3)
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阳性对照
- Whole cell lysate from 293T cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70254于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at 1-5 µg/mg of lysate.
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说明 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 1-5 µg/mg of lysate. |
靶标
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功能
Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints. -
组织特异性
Highly expressed in testis. -
发展阶段
Expression peaks in late G2/S phase of the cell cycle. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Chromosome > telomere. Cytoplasm > cytoskeleton > spindle. Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase. - Information by UniProt
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数据库链接
- Entrez Gene: 459657 Chimpanzee
- Entrez Gene: 55183 Human
- Omim: 608952 Human
- SwissProt: Q5UIP0 Human
- Unigene: 655671 Human
- Unigene: 725538 Human
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别名
- DKFZp781N1478 antibody
- FLJ10599 antibody
- FLJ11269 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryngeal squamous cell carcinoma tissue labelling Rif1 with ab70254 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation analysis of 293T whole cell lysate (1mg for IP, 25% of reaction loaded), using ab70254 at 5 µg/mg lysate for IP. Immunoprecipitated Rif1 was blotted using ab70307 at 0.4µg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab70254 被引用在 2 文献中.
- Hiraga SI et al. Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation. EMBO Rep 18:403-419 (2017). IP ; Human . PubMed: 28077461
- Alver RC et al. Reversal of DDK-Mediated MCM Phosphorylation by Rif1-PP1 Regulates Replication Initiation and Replisome Stability Independently of ATR/Chk1. Cell Rep 18:2508-2520 (2017). PubMed: 28273463