Application
Immunocytochemistry/ Immunofluorescence
Blocking step
PBS-1% ovalbumin as blocking agent for 20 minute(s) · Concentration: 1.0% · Temperature: 22°C
Sample
Salmo salar Cell (testicular)
Specification
testicular
Permeabilization
Yes - methyl methacrylate/Butyl methacrylate
Fixative
glutaraldehyde-formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS-1% ovalbumin
Dilution
1/80
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFluor 568 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Dilution
1/100
Additional data
Additional Notes
ABTRIAL-EV7ZK
Abcam response
Thank you for your valuable feedback. We have not previously tested this antibody in Salmo salar and therefore it may not be suitable for use in this species. Further optimization may improve results.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 25 2015