Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - other (Ribosome enriched sample)
Loading amount
20 µg
Specification
Ribosome enriched sample
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1XTBS, 0.1% Tween-20 with 5% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG HRP-linked Antibody (Cell Signalin
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: None kDa Non-specific: 23, 30, 37, 65, 75, kDa
Positive control
N/A
Negative control
N/A
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Joseph Moore Iv
Verified customer
提交于 Dec 21 2009