Anti-Ribonuclease H2,subunit A/RNASEH2A抗体(ab92876)
Key features and details
- Mouse polyclonal to Ribonuclease H2, subunit A/RNASEH2A
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Ribonuclease H2,subunit A/RNASEH2A抗体
参阅全部 Ribonuclease H2, subunit A/RNASEH2A 一抗 -
描述
小鼠多克隆抗体to Ribonuclease H2,subunit A/RNASEH2A -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human Ribonuclease H2, subunit A/RNASEH2A. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_006388.2 -
阳性对照
- COLO 320 HSR cell lysate, Jurkat cell lysate and HeLa cell.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92876于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 33 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 33 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes. -
疾病相关
Aicardi-Goutieres syndrome 4 -
序列相似性
Belongs to the RNase HII family. Eukaryotic subfamily. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10535 Human
- SwissProt: O75792 Human
- Unigene: 532851 Human
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别名
- AGS4 antibody
- Aicardi Goutieres syndrome 4 protein antibody
- Aicardi-Goutieres syndrome 4 protein antibody
see all
图片
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Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab92876) at 1/500 dilution + COLO 320 HSR at 50 µg
Predicted band size: 33 kDa -
Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab92876) at 1/500 dilution + Jurkat cell lysate at 50 µg
Predicted band size: 33 kDa -
All lanes : Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab92876) at 1/500 dilution
Lane 1 : Ribonuclease H2, subunit A/RNASEH2A transfected 293T cell lysate
Lane 2 : Non-transfected cell line.
Lysates/proteins at 50 µg per lane.
Predicted band size: 33 kDa -
ab92876 at 10µg/ml staining Ribonuclease H2, subunit A/RNASEH2A on Hela cell by immunofluorescence
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab92876 被引用在 3 文献中.
- Zimmermann M et al. CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Nature 559:285-289 (2018). PubMed: 29973717
- Kennedy EM et al. Frequent Incorporation of Ribonucleotides during HIV-1 Reverse Transcription and Their Attenuated Repair in Macrophages. J Biol Chem 287:14280-8 (2012). WB . PubMed: 22383524
- Helmrich A et al. Collisions between replication and transcription complexes cause common fragile site instability at the longest human genes. Mol Cell 44:966-77 (2011). PubMed: 22195969