Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T)
Total protein in input
1000 µg
Specification
293T
Treatment
Transfected Flag-tag TNFa protein with Fugene
Immuno-precipitation step
Protein A/G
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 25mM Tris PH 7.4 150mM NaCl 1mM EDTA
Western Blot antibody
Name
Abcam antibody:(not specified yet)
Additional data
Abcam response
Thank you for your review of this untested application. We value the feedback gathered during your studies and appreciate that you have shared it with us and our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 May 07 2013