Application
Western blot
Sample
Pig Cell lysate - whole cell (Primary brain endothelial cells)
Gel Running Conditions
Reduced Denaturing (10% resolving gel)
Loading amount
10 µg
Specification
Primary brain endothelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% (w/v) milk in TBST
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG-HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
no
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 51 kDa
Positive control
Rat Liver
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Yu Siong Ho
Verified customer
提交于 Oct 23 2017