Application
Western blot
Sample
Zebrafish Tissue lysate - whole (load 1 embryo @ 4dpf)
Loading amount
1 µg
Specification
load 1 embryo @ 4dpf
Treatment
inject GFP-Reptin mRNA @ 1 cell stage
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: HRP-anti Rabbit light chain
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 75 kDa Non-specific: 100, 70, 50 kDa
Positive control
GFP-Reptin overexpressed embryo lysate blotted with anti-GFP antibody
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 23 2010