Anti-Reptin/TIP49B/RUVB2抗体(ab89942)
Key features and details
- Mouse polyclonal to Reptin/TIP49B/RUVB2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Reptin/TIP49B/RUVB2抗体
参阅全部 Reptin/TIP49B/RUVB2 一抗 -
描述
小鼠多克隆抗体to Reptin/TIP49B/RUVB2 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human Reptin/TIP49B/RUVB2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- Human pancreas tissue lysate, HeLa whole cell lysate (ab150035), Reptin/TIP49B/RUVB2 transfected 293T cell lysate, HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab89942于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. -
组织特异性
Ubiquitously expressed. Highly expressed in testis and thymus. -
序列相似性
Belongs to the ruvB family. -
结构域
The C-terminal domain is required for association with ATF2. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes. - Information by UniProt
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数据库链接
- Entrez Gene: 10856 Human
- Omim: 604788 Human
- SwissProt: Q9Y230 Human
- Unigene: 515846 Human
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别名
- 48 kDa TATA box-binding protein-interacting protein antibody
- 48 kDa TBP-interacting protein antibody
- 48-kDa TATA box-binding protein-interacting protein antibody
see all
图片
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Anti-Reptin/TIP49B/RUVB2 antibody (ab89942) at 1 µg/ml + Human pancreas tissue lysate at 50 µg
Predicted band size: 51 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted? -
Anti-Reptin/TIP49B/RUVB2 antibody (ab89942) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 51 kDa
Observed band size: 51 kDa -
All lanes : Anti-Reptin/TIP49B/RUVB2 antibody (ab89942) at 1 µg/ml
Lane 1 : Reptin/TIP49B/RUVB2 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa -
ab89942 at 10 µg/ml staining Reptin/TIP49B/RUVB2 in HeLa cells by Immunofluorescence.
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab89942 被引用在 2 文献中.
- Runge JS et al. Identification of Two Distinct Classes of the Human INO80 Complex Genome-Wide. G3 (Bethesda) 8:1095-1102 (2018). PubMed: 29432129
- Cho KJ et al. ZMYND10 stabilizes intermediate chain proteins in the cytoplasmic pre-assembly of dynein arms. PLoS Genet 14:e1007316 (2018). PubMed: 29601588