Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse cell lines)
Loading amount
20 µg
Specification
Mouse cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Other product details
Concentration
1 µg/ml
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA, PBS-Tween 0.1%
Secondary antibody
Name
Non-Abcam antibody was used: Swine anti Rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
Supersignal west dura
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 57, (35?) kDa
Additional data
Additional Notes
Order: (1) MEF, (2) F9, (3) GC-1, (4) NIH-3T3
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 22 2012