Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal Muscle)
Loading amount
50 µg
Specification
Skeletal Muscle
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Concentration
1 µg/ml
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 second(s)
Bands
Specific: 51 kDa Non-specific: 48 kDa
Additional data
Abcam response
Thank you for your valuable feedback. We are pleased to recieve this is the first data using this antibody in rat samples. This antibody has detected a band at the correct size of 51 kDa, but also an intense band at 48 kDa. We notice this experiment has been run under non reducing conditions. It may be that under non reducing condition, the protein is visible in two different forms observed at different molecular weights. We would be pleased to recieve any further data from customers who have used this antibody in rat to confirm what the results would be under reducing conditions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Carlos Castorena
Verified customer
提交于 Mar 22 2010