重组大鼠BVR蛋白(ab91622)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
描述
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产品名称
重组大鼠BVR蛋白
参阅全部 BVR 蛋白酶 -
生物活性
BVR Assay: Positive (> 180 units/mg) 1 unit of BVR will transform 1 nanomole of biliverdin to bilirubin in an NADPH dependent reaction at pH 8.5 at 37°C in 1 minute.
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纯度
> 95 % SDS-PAGE.
Purified by Multi-Step Chromatography. > 95% pure as determined by SDS-PAGE and Western Blot analysis. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Rat
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技术指标
Our Abpromise guarantee covers the use of ab91622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
BVR Assay: Positive (> 180 units/mg) 1 unit of BVR will transform 1 nanomole of biliverdin to bilirubin in an NADPH dependent reaction at pH 8.5 at 37°C in 1 minute.
Protein previously labeled as Biliverdin Reductase.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.79% Tris HCl, 0.0292% EDTA
This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- BIEA_HUMAN
- Biliverdin IX alpha reductase
- Biliverdin reductase A
see all -
功能
Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor. -
组织特异性
Liver. -
通路
Porphyrin-containing compound metabolism; protoheme degradation. -
疾病相关
Hyperbiliverdinemia -
序列相似性
Belongs to the Gfo/Idh/MocA family. Biliverdin reductase subfamily. -
细胞定位
Cytoplasm. - Information by UniProt
图片
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SDS-PAGE Analysis:
Lane 1: Molecular weight marker Lane 2: 0.5 µg ab91622
Lane 3: 1.0 µg ab91622 Lane 4: 2.0 µg ab91622
Lane 5: 5.0 µg ab91622 -
All lanes : BVR Polyclonal Antibody
Lane 1 : Molecular weight markers
Lane 2 : ab91622 at 0.1 µg -
BVR Activity: 1 µg of ab91622 was incubated with biliverdin in the presence of NADPH at pH 8.5, 37°C. Product formation was monitored with a spectrometer at 450 nm.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab91622 尚未被引用在任何文献中。