重组小鼠Wnt3a蛋白(ab81484)
Key features and details
- Expression system: Cell Culture
- Purity: > 75% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: CellDiff, SDS-PAGE, Cellular Activation, HPLC
描述
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产品名称
重组小鼠Wnt3a蛋白
参阅全部 Wnt3a 蛋白酶 -
生物活性
The ED50 was determined by its ability to induced alkaline phosphatase production by CCL-226 cells.
The expected ED50 for this effect is 0.4-0.6 ng/ml.
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纯度
> 75 % SDS-PAGE.
Greater than 75% by HPLC analyses. -
内毒素水平
< 1.000 Eu/µg -
表达系统
Cell Culture -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
序列
SY PIWWSLAVGP QYSSLSTQPI LCASIPGLVP KQLRFCRNYV EIMPSVAEGV KAGIQECQHQ FRGRRWNCTT VSNSLAIFGP VLDKATRESA FVHAIASAGV AFAVTRSCAE GSAAICGCSS RLQGSPGEGW KWGGCSEDIE FGGMVSREFA DARENRPDAR SAMNRHNNEA GRQAIASHMH LKCKCHGLSG SCEVKTCWWS QPDFRTIGDF LKDKYDSASE MVVEKHRESR GWVETLRPRY TYFKVPTERD LVYYEASPNF CEPNPETGSF GTRDRTCNVS SHGIDGCDLL CCGRGHNART ERRREKCHCV FHWCCYVSCQ ECTRVYDVHT CK -
氨基酸
19 to 352 -
额外的序列信息
ab81484 is a monomeric glycoprotein containing 334 amino acid residues. This protein is the mature full length (aa19-352), it does not contain the signal peptide from aa 1-18.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab81484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Cell Differentiation
SDS-PAGE
Cellular Activation
HPLC
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形式
Lyophilized -
补充说明
Endotoxin level is less than 0.2 ng per µg (2EU/µg).
Formulation: Sterile filtered through a 0.2 micron filter. Lyophilized from 10mM Sodium Phosphate, pH 7.5 + 500mM NaCl + 0.5% CHAPS.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at 4°C (up to 6 months). The lyophilized protein is stable for a few weeks at room temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 13% CHAPS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute to a concentration of 0.1 mg/mL with ddH2O. Store at 4oC for up to 1 week or dilute further in a buffer containing a carrier protein or stabilizer and store working aliquots at -20oC to -80oC for up to 3 months.
常规信息
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别名
- Protein Wnt 3a Precursor
- Protein Wnt-3a
- Wingless type MMTV integration site family member 3A
see all -
功能
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. -
组织特异性
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. -
序列相似性
Belongs to the Wnt family. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
图片
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Human embryonic stem cells (HUES4) were differentiated as monolayer cultures on matrigel coated dishes using a protocol described in Kroon et al, Nat Biotechnol. (2008). Inhibin beta A (ab50051) and Wnt3a (ab81484) were added to cultures to direct differentiation towards definitive endoderm. This image was taken 3 days after induction, where the definitive endoderm marker FOXA2 (ab40874 in green) is abundant. Dapi staining is shown in blue. Magnification: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab81484 被引用在 15 文献中.
- Balbasi E et al. Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages. Methods Mol Biol 2520:295-307 (2022). PubMed: 34611822
- Cheng K et al. Targeted intestinal deletion of Rho guanine nucleotide exchange factor 7, ßPIX, impairs enterocyte proliferation, villus maturation, and mucosal defenses in mice. Am J Physiol Gastrointest Liver Physiol 320:G627-G643 (2021). PubMed: 33566751
- Liu B et al. Loss of endothelial glucocorticoid receptor promotes angiogenesis via upregulation of Wnt/ß-catenin pathway. Angiogenesis 24:631-645 (2021). PubMed: 33650028
- Gong Q et al. Fractalkine aggravates LPS-induced macrophage activation and acute kidney injury via Wnt/ß-catenin signalling pathway. J Cell Mol Med 25:6963-6975 (2021). PubMed: 34101346
- Zhou C et al. LncRNA PVT1 promotes gemcitabine resistance of pancreatic cancer via activating Wnt/ß-catenin and autophagy pathway through modulating the miR-619-5p/Pygo2 and miR-619-5p/ATG14 axes. Mol Cancer 19:118 (2020). PubMed: 32727463