重组小鼠PKM2蛋白(ab95474)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组小鼠PKM2蛋白
参阅全部 PKM2 蛋白酶 -
生物活性
Specific Activity: 11 pmol/min/µg. A 50 µl PKM2 reaction is conducted in a buffer containing 50 mM Tris (pH 7.4), 100mM MgCl2, 250 mM KCl, 40 mM ADP, 100 mM phosphoenolpyruvate (PEP) at room temperature for 15 min. ATP production is detected using Kinase-Glo Luminescent Kinase Assay Platform.
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纯度
> 90 % SDS-PAGE. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
氨基酸
2 to 531
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab95474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.36% Imidazole
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.63% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.642% Sodium chloride, 0.016% Potassium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- CTHBP
- Cytosolic thyroid hormone binding protein
- Cytosolic thyroid hormone-binding protein
see all -
功能
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. -
组织特异性
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. -
通路
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. -
序列相似性
Belongs to the pyruvate kinase family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
细胞定位
Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab95474 尚未被引用在任何文献中。