重组小鼠GDNF蛋白(ab56286)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组小鼠GDNF蛋白
参阅全部 GDNF 蛋白酶 -
生物活性
Biological Activity : The ED50 was determined by the proliferation of rat C6 cells is = 0.2 ng/ml, corresponding to a specific activity of = 5 x 106 units/mg.
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纯度
> 95 % SDS-PAGE.
Endotoxin level is less than 0.1 ng per µg (1EU/µg). -
表达系统
Escherichia coli -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
序列
MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI -
氨基酸
79 to 211
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技术指标
Our Abpromise guarantee covers the use of ab56286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstituted GDNF is stable for at least 3 months when stored in working aliquots with a carrier protein at -20oC.
常规信息
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别名
- Astrocyte derived trophic factor
- Astrocyte derived trophic factor 1
- Astrocyte-derived trophic factor
see all -
功能
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. -
组织特异性
In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. -
疾病相关
Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. -
序列相似性
Belongs to the TGF-beta family. GDNF subfamily. -
细胞定位
Secreted. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (2)
ab56286 被引用在 2 文献中.
- Klimovich P et al. Three-Dimensional Model of Dorsal Root Ganglion Explant as a Method of Studying Neurotrophic Factors in Regenerative Medicine. Biomedicines 8:N/A (2020). PubMed: 32138155
- Rota C et al. Human amniotic fluid stem cell preconditioning improves their regenerative potential. Stem Cells Dev : (2011). PubMed: 22066606