重组小鼠EGF蛋白(ab72994)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组小鼠EGF蛋白
参阅全部 EGF 蛋白酶 -
生物活性
The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml.
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纯度
> 95 % SDS-PAGE.
Purity is greater than 97% as determined by reducing and non-reducing SDS-PAGE and analytical HPLC. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
序列
NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR
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技术指标
Our Abpromise guarantee covers the use of ab72994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with sterile water at a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions. Reconstituted material should be aliquoted and frozen at -20oC. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
常规信息
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别名
- Beta urogastrone
- beta-urogastrone
- EGF
see all -
功能
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). -
组织特异性
Expressed in kidney, salivary gland, cerebrum and prostate. -
疾病相关
Hypomagnesemia 4 -
序列相似性
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats. -
翻译后修饰
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). -
细胞定位
Membrane. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab72994 被引用在 1 文献中.
- Peng DH et al. Epidermal growth factor alleviates cerebral ischemia-induced brain injury by regulating expression of neutrophil gelatinase-associated lipocalin. Biochem Biophys Res Commun 524:963-969 (2020). PubMed: 32059851