重组人WISP1蛋白(ab50041)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人WISP1蛋白 -
生物活性
Biological Activity : The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0-3.0 µg/ml.
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纯度
> 98 % SDS-PAGE.
Greater than 98% by and HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g). -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
TALSPAPTTM DFTPAPLEDT SSRPQFCKWP CECPPSPPRC PLGVSLITDG CECCKMCAQQ LGDNCTEAAI CDPHRGLYCD YSGDRPRYAI GVCAQVVGVG CVLDGVRYNN GQSFQPNCKY NCTCIDGAVG CTPLCLRVRP PRLWCPHPRR VSIPGHCCEQ WVCEDDAKRP RKTAPRDTGA FDAVGEVEAW HRNCIAYTSP WSPCSTSCGL GVSTRISNVN AQCWPEQESR LCNLRPCDVD IHTLIKAGKK CLAVYQPEAS MNFTLAGCIS TRSYQPKYCG VCMDNRCCIP YKSKTIDVSF QCPDGLGFSR QVLWINACFC NLSCRNPNDI FADLESYPDF SEIAN
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技术指标
Our Abpromise guarantee covers the use of ab50041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.
Constituent: 0.06% Acetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute product with 10mM Acetic Acid.
常规信息
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别名
- CCN 4
- CCN family member 4
- CCN4
see all -
相关性
WISP1 is a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival. WISP1 attentuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X(L) protein. WISP1 is expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Overexpression is associated with several cancers including breast cancer and colon tumors. -
细胞定位
Secreted
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab50041 被引用在 3 文献中.
- Rong X et al. Human bone marrow mesenchymal stem cells-derived exosomes alleviate liver fibrosis through the Wnt/ß-catenin pathway. Stem Cell Res Ther 10:98 (2019). PubMed: 30885249
- Meyers CA et al. WISP-1 drives bone formation at the expense of fat formation in human perivascular stem cells. Sci Rep 8:15618 (2018). PubMed: 30353078
- Li WF et al. WISP-1 contributes to fractionated irradiation-induced radioresistance in esophageal carcinoma cell lines and mice. PLoS One 9:e94751 (2014). ICC/IF ; Mouse, Human . PubMed: 24728101