Recombinant人ULK3 protein (ab101548)

概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 分子量
      51 kDa including tags
    • 标签
      His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab101548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    The Specific activity of ab101548 was determined to be 230 nmol/min/mg.
  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度
    > 95 % SDS-PAGE.
    The purity was determined to be >95% by densitometry
  • 形式
    Liquid
  • 补充说明

    ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.25mM DTT, 0.1mM PMSF, pH 7.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • DKFZP434C131
    • FLJ90566
    • Serine/threonine-protein kinase ULK3
    • Ulk3
    • ULK3_HUMAN
    • unc 51 like kinase 3 (C. elegans)
    • Unc-51-like kinase 3
    see all
  • 功能
    Serine/threonine protein kinase which enhances GLI1 and GLI2 transcriptional activity and consequently positively regulates GLI-dependent SHH signaling. May exert this function by promoting GLI1 nuclear localization. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.
  • 组织特异性
    Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optice nerve. In the central nervous system, lowest levels in the spinal cord.
  • 序列相似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
    Contains 2 MIT domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Autophosphorylated in vitro.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt

图片

  • SDS-PAGE showing ab101548 at approximately 51kDa.
  • Kinase Assay demonstrating specific activity of ab101548.

文献

ab101548 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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