重组人Transferrin蛋白(ab83560)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
-
产品名称
重组人Transferrin蛋白
参阅全部 Transferrin 蛋白酶 -
生物活性
The activity of ab83560 was measured by its ability to support the growth of HepG2 cells under conditions of reduced serum. Typically 0.1 to 1 µg enhances cell proliferation.
-
纯度
> 95 % SDS-PAGE. -
表达系统
HEK 293 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
序列
Theoretical sequence: VPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASY L DCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKED PQTF YYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLL YCDLPEP RKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCS TLNQYFGYSG AFKCLKNGAGDVAFVKHSTIFENLANKADRDQYEL LCLDNTRKPVDEY KDCHLAQVPSHTVVARSMGGKEDLIWELLNQA QEHFGKDKSKEFQLFS SPHGKDLLFKDSAHGFLKVPPRMDAKMYL GYEYVTAIRNLREGTCQEA PTDECKPVKWCALSHHERLKCDEWSV NSVGKIECVSAETTEDCIAKIM NGEADAMSLDGGFVYIAGKCGLV PVLAENYNKSDNCEDTPEAGYFAVA VVKKSASDLTWDNLKGKKSC HTAVGRTAGWNIPMGLLYNKINHCRFDE FFSEGCAPGSKKDSSLC KLCMGSGLNLCEPNNKEGYYGYTGAFRCLVE KGDVAFVKHQTVPQ NTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEY ANCHLARAPNH AVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLF RSETKDLL FRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSL LEACT FRRP
-
相关产品
-
Affibody® Molecule
-
Related Products
技术指标
Our Abpromise guarantee covers the use of ab83560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Functional Studies
SDS-PAGE
-
形式
Lyophilized -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
复溶It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
常规信息
-
别名
- Apotransferrin
- Beta 1 metal binding globulin
- Beta-1 metal-binding globulin
see all -
功能
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. -
组织特异性
Expressed by the liver and secreted in plasma. -
疾病相关
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. -
序列相似性
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
细胞定位
Secreted. - Information by UniProt
图片
-
Lane 1 – MW markers; Lane 2 – ab83560; Lane 3 – ab83560 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83560 treated with a glycosidase cocktail to remove potential N- and Olinked glycans. Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie.
Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes. -
A sample of ab83560 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris HCl 2D gel. Approximately 40 μg of protein was load; Gel was stained using Deep Purple™. Spot train indicates presence of multiple isoforms of Transferrin. Spots within the spot train were cut from the gel and identified as Transferrin by protein mass fingerprinting.
-
Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates that ab83560 exists in multiple isoforms, which differ according to their level of posttranslational modification. Expression of these isoforms is highly significant for cell biology, as they more closely resemble the native human proteins. The triangle indicates theoretical pI and MW of the protein.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (0)
ab83560 尚未被引用在任何文献中。