重组人TGF beta 2蛋白(Active) (ab84070)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, Functional Studies, SDS-PAGE, WB
Achieve higher consistency and quality standards with a premium grade bioactive protein
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
描述
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产品名称
重组人TGF beta 2蛋白(Active)
参阅全部 TGF beta 2 蛋白酶 -
生物活性
Determined by its ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells.
The ED50 was found to be = 0.2 ng/ml, corresponding to a specific activity of = 5 x 106 units/mg.
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纯度
> 98 % SDS-PAGE.
> 98% HPLC analyses. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
ALDAAYCFRN VQDNCCLRPL YIDFKRDLGW KWIHEPKGYN ANFCAGACPY LWSSDTQHSR VLSLYNTINP EASASPCCVS QDLEPLTILY YIGKTPKIEQ LSNMIVKSCK CS -
预测分子量
25 kDa -
氨基酸
303 to 414
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技术指标
Our Abpromise guarantee covers the use of ab84070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
HPLC
Functional Studies
SDS-PAGE
Western blot
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Stable for 12 months at -20°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute in water to 0.1-1.0 mg/ml. Store at 2°C to 8°C for up to 1 week or prepare for extended storage. After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20°C to -80°C.
常规信息
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别名
- BSC-1 cell growth inhibitor
- BSC1 cell growth inhibitor
- Cetermin
see all -
功能
TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. -
疾病相关
A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with HDAC9.
Loeys-Dietz syndrome 4
Defects in TGFB2 may be a cause of non-syndromic aortic disease (NSAD). NSAD is a frequently asymptomatic but potentially lethal disease characterized by thoracic aortic aneurysms and dissections without additional syndromic features. -
序列相似性
Belongs to the TGF-beta family. -
翻译后修饰
The precursor is cleaved into mature TGF-beta-2 and LAP, which remains non-covalently linked to mature TGF-beta-2 rendering it inactive. -
细胞定位
Secreted. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (8)
ab84070 被引用在 8 文献中.
- Li Q et al. Naringenin inhibits autophagy and epithelial-mesenchymal transition of human lens epithelial cells by regulating the Smad2/3 pathway. Drug Dev Res 83:389-396 (2022). PubMed: 34402084
- Spurlin JW et al. BMP3 inhibits TGFβ2-mediated myofibroblast differentiation during wound healing of the embryonic cornea. NPJ Regen Med 7:36 (2022). PubMed: 35879352
- Jie X et al. USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-ß2 transcription. Cell Death Differ 28:2095-2111 (2021). PubMed: 33558705
- Finger AM et al. Intercellular coupling between peripheral circadian oscillators by TGF-ß signaling. Sci Adv 7:N/A (2021). PubMed: 34301601
- Corbet C et al. TGFß2-induced formation of lipid droplets supports acidosis-driven EMT and the metastatic spreading of cancer cells. Nat Commun 11:454 (2020). PubMed: 31974393
- Babushkina A & Lwigale P Periocular neural crest cell differentiation into corneal endothelium is influenced by signals in the nascent corneal environment. Dev Biol 465:119-129 (2020). PubMed: 32697973
- Tran DQ et al. GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells. Proc Natl Acad Sci U S A 106:13445-50 (2009). PubMed: 19651619
- O'Riordan E et al. Chronic NOS inhibition actuates endothelial-mesenchymal transformation. Am J Physiol Heart Circ Physiol 292:H285-94 (2007). PubMed: 16963618