重组人PPM1A蛋白(ab51205)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人PPM1A蛋白
参阅全部 PPM1A 蛋白酶 -
生物活性
Specific activity: > 8,000units/mg of PPM1A. Unit Definition - one unit will hydrolyze 1 nanomole of p-nitrophenylphosphatate per minute at pH 7.4 at 37°C using 10mM of substrate. -
纯度
> 95 % SDS-PAGE.
ab51205 was purified by conventional column chromatography. -
内毒素水平
< 1.000 Eu/µg -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW -
氨基酸
1 to 382
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技术指标
Our Abpromise guarantee covers the use of ab51205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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质谱法
MALDI-TOF -
形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.0308% DTT, 0.158% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.29% Sodium chloride, 0.0126% Manganese chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Protein phosphatase 2C isoform alpha
- EC 3.1.3.16
- FLJ42306
see all -
功能
Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. -
序列相似性
Belongs to the PP2C family. -
细胞定位
Nucleus. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab51205 尚未被引用在任何文献中。