重组人JAK3蛋白(Active) (ab89982)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
-
产品名称
重组人JAK3蛋白(Active)
参阅全部 JAK3 蛋白酶 -
生物活性
Specific Activity: 500 pmol/min/μg. Assay conditions: 40 mM Tris-HCL pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 0.2 mM DTT using 0.2 mg/ml Poly-(Glu4:Tyr) substrate and 20 mM ATP. Reaction was done at 30°C for 45 min.
-
纯度
>= 90 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected insect cells -
Accession
-
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
实际分子量
37 kDa including tags -
氨基酸
811 to 1124 -
标签
His tag N-Terminus -
额外的序列信息
NM_000215. N-terminal 10x His tag.
-
技术指标
Our Abpromise guarantee covers the use of ab89982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Functional Studies
SDS-PAGE
-
形式
Liquid -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on Dry Ice. Store at -80°C.
pH: 8.00
Preservative: 1.36% Imidazole
Constituents: 0.63% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
别名
- EC 2.7.10.2
- JAK 3
- JAK L
see all -
功能
Tyrosine kinase of the non-receptor type, involved in the interleukin-2 and interleukin-4 signaling pathway. Phosphorylates STAT6, IRS1, IRS2 and PI3K. -
组织特异性
In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins. -
疾病相关
Defects in JAK3 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-negative (T(-)B(+)NK(-) SCID) [MIM:600802]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain. -
结构域
Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1. -
翻译后修饰
Tyrosine phosphorylated in response to IL-2 and IL-4. -
细胞定位
Endomembrane system. Wholly intracellular, possibly membrane associated. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab89982 尚未被引用在任何文献中。