重组人IKK alpha蛋白(Active) (ab102103)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB, Functional Studies
描述
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产品名称
重组人IKK alpha蛋白(Active) -
生物活性
Specific activity is 2.5nm/min/mg
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纯度
> 75 % Densitometry.
ab102103 was determined to be >75% pure by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
分子量信息
Approx 114 kDa by SDS-PAGE -
氨基酸
1 to 745 -
标签
GST tag N-Terminus -
额外的序列信息
GenBank: BC092514
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相关产品
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Related Products
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab102103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
Functional Studies
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形式
Liquid -
补充说明
ab204863 (IKB alpha peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- chuk
- CHUK1
- Conserved Helix Loop Helix Ubiquitous Kinase
see all -
功能
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines. -
组织特异性
Widely expressed. -
疾病相关
Defects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway. -
细胞定位
Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus. - Information by UniProt
图片
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The specific activity of IKK alpha (ab102103) was determined to be 2.9 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab102103
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SDS PAGE analysis of ab102103
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ab102103 was determined to be >75% pure by densitometry. MWt ~114 kDa.
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The specific activity of ab102103 was determined to be 2.5 nmol/mg/min.
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Anti-IKK alpha antibody [Y463] (ab32041) at 1/10000 dilution +
Recombinant human IKK alpha protein (Active) (ab102103) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab102103 被引用在 1 文献中.
- Colomer C et al. IKKa Kinase Regulates the DNA Damage Response and Drives Chemo-resistance in Cancer. Mol Cell 75:669-682.e5 (2019). PubMed: 31302002