重组人Hsp70蛋白(ab78427)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, WB, ELISA, SDS-PAGE
描述
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产品名称
重组人Hsp70蛋白
参阅全部 Hsp70 蛋白酶 -
生物活性
ab78427 has ATPase activity at the time of manufacture of 3.3µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
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纯度
> 90 % SDS-PAGE.
ab78427 is affinity purified. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MAKAAAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFQVINDGDK PKVQVSYKGE TKAFYPEEIS SMVLTKMKEI AEAYLGYPVT NAVITVPAYF NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT KDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK ITITNDKGRL SKEEIERMVQ EAEKYKAEDE VQRERVSAKN ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE QVCNPIISGL YQGAGGPGPG GFGAQGPKGG SGSGPTIEEV D -
标签
His tag N-Terminus
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相关产品
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Related Products
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Hsp70 antibody [5A5] (ab2787)
- Anti-Hsp70 antibody (ab31010)
- Anti-Hsp70 antibody [EP1007Y] (ab45133)
- Anti-Hsp70 antibody [C92F3A-5] (ab47455)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Hsp70 antibody [3A3] (ab5439)
- Anti-Hsp70 antibody [2A4] (ab5442)
技术指标
Our Abpromise guarantee covers the use of ab78427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
Western blot
ELISA
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 1.36% Imidazole
Constituents: 0.87% Sodium chloride, 10% Glycerol (glycerin, glycerine), 0.328% Sodium phosphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- DnaK type molecular chaperone HSP70 1
- Epididymis secretory protein Li 103
- FLJ54303
see all -
相关性
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific. -
细胞定位
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab78427 尚未被引用在任何文献中。