重组人Eph receptor A1/EphA1蛋白(ab101778)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 90% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies
描述
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产品名称
重组人Eph receptor A1/EphA1蛋白
参阅全部 Eph receptor A1/EphA1 蛋白酶 -
生物活性
The Specific activity of ab101778 was determined to be 28 nmol/min/mg.
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纯度
> 90 % Densitometry.
Purity was determined to be >90% by densitometry. Affinity purified. -
表达系统
Baculovirus infected insect cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
72 kDa including tags -
氨基酸
569 to 976
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab101778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
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形式
Liquid -
补充说明
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
Previously labelled as Eph receptor A1.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- EPH
- EPH receptor A1
- EPH tyrosine kinase
see all -
功能
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis. -
组织特异性
Overexpressed in several carcinomas. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 1 Eph LBD (Eph ligand-binding) domain.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
翻译后修饰
Phosphorylated. Autophosphorylation is stimulated by its ligand EFNA1.
Ubiquitinated. -
细胞定位
Cell membrane. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab101778 尚未被引用在任何文献中。