重组人Caspase-6/CASP-6蛋白(ab52157)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人Caspase-6/CASP-6蛋白
参阅全部 Caspase-6/CASP-6 蛋白酶 -
生物活性
SPECIFIC ACTIVITY: 13,000 units/mg
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纯度
> 95 % SDS-PAGE. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MSSASGLRRGHPAGGEENMTETDAFYKREMFDPAEKYKMDHRRRGIALIF NHERFFWHLTLPERRGTCADRDNLTRRFSDLGFEVKCFNDLKAEELLLKI HEVSTVSHADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTGLFKGDKCHS LVGKPKIFIIQACRGNQHDVPVIPLDVVDNQTEKLDTNITEVDAASVYTL PAGADFLMCYSVAEGYYSHRETVNGSWYIQDLCEMLGKYGSSLEFTELLT LVNRKVSQRRVDFCKDPSAIGKKQVPCFASMLTKKLHFFPKSN
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab52157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Lyophilized -
补充说明
This product is manufactured by BioVision, an Abcam company and was previously called 1086 Caspase-6, human recombinant. 1086-100 is the same size as the 100 unit size of ab52157.
UNIT DEFINITION: One unit of the recombinant Caspase-6 / CASP-6 is the enzyme activity that cleaves 1 nmol of the caspase substrate VEID-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: PBS, 15% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute to 1 unit per µl in PBS containing 15% glycerol. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –80°C.
常规信息
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别名
- Apoptotic protease Mch-2
- Apoptotic protease MCH2
- CASP-6
see all -
功能
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. -
序列相似性
Belongs to the peptidase C14A family. -
翻译后修饰
Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits. -
细胞定位
Cytoplasm. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab52157 被引用在 3 文献中.
- Hung MC et al. AKT phosphorylation as a predictive biomarker for PI3K/mTOR dual inhibition-induced proteolytic cleavage of mTOR companion proteins in small cell lung cancer. Cell Biosci 12:122 (2022). PubMed: 35918763
- Zandy AJ et al. Role of the executioner caspases during lens development. J Biol Chem 280:30263-72 (2005). PubMed: 15994297
- Basu A et al. Proteolytic activation of protein kinase C-epsilon by caspase-mediated processing and transduction of antiapoptotic signals. J Biol Chem 277:41850-6 (2002). PubMed: 12198125