重组Mycobacterium tuberculosis TB Ag85A蛋白(ab124604)
Key features and details
- Expression system: Baculovirus infected BTI-TN-5B1-4 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组Mycobacterium tuberculosis TB Ag85A蛋白
参阅全部 TB Ag85A 蛋白酶 -
纯度
> 95 % SDS-PAGE.
Affinity purified -
内毒素水平
< 1.000 Eu/µg -
表达系统
Baculovirus infected BTI-TN-5B1-4 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mycobacterium tuberculosis -
序列
ADPAFSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGAHHHHHH -
预测分子量
33 kDa including tags -
氨基酸
44 to 338 -
标签
His tag C-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab124604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine)
常规信息
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别名
- 85 A
- 85A
- Ag85A
see all -
相关性
Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. These proteins are responsible for the high affinity of mycobacteria to fibronectin. All three proteins possesses a mycolyltransferase activity - which is required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Thus all three proteins are involved in the final stages of mycobacterial cell wall assembly. -
细胞定位
Secreted; cell wall. Cytoplasm
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab124604 被引用在 1 文献中.
- Mancha-Agresti P et al. Recombinant Invasive Lactococcus lactis Carrying a DNA Vaccine Coding the Ag85A Antigen Increases INF-?, IL-6, and TNF-a Cytokines after Intranasal Immunization. Front Microbiol 8:1263 (2017). Functional Studies ; Mouse . PubMed: 28744263