Application
Western blot
Review text: p53 protein (100&400 ng) was anylysed by SDS-PAGE electrophoresis (10% Tris-Gly gel) and Western Blotting with Abcam p53 antibodies (ab26)
Sample: Human Purified protein
Loading amount: 0.1 µg
Gel Running Conditions: Reduced Denaturing (10% Tris-Gle gel)
Blocking step: LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Other product details
Incubation time: 2 hour(s) and 0 minute(s) · Diluent: Odyssey® Blocker 1:1 in PBS + 0.1% Tween
Primary antibody (in addition to 'p53 protein (Tagged)')
Primary antibody: Abcam primary antibody: Anti-p53 antibody [PAb 240] (ab26)
Dilution: 1/2000
Secondary antibody
Name: Non-Abcam antibody was used: IR Dye 800 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution: 1/10000
Detection
Detection method: Odyssey® scanner
Exposure: 3 minute(s) and 0 second(s)
Bands: Specific: 75 kDa Non-specific: - kDa
Positive control: p53 purified protein
Negative control: -
Additional data
Additional Notes: Picture legend.
100ng (1) or 400ng (2) of purified protein loaded
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 29 2010