重组人PHLPP2蛋白(ab125556)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% Densitometry
- Suitable for: WB, SDS-PAGE
描述
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产品名称
重组人PHLPP2蛋白 -
纯度
> 95 % Densitometry.
ab125556 is >95% pure. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
56 kDa including tags -
氨基酸
766 to 1043
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技术指标
Our Abpromise guarantee covers the use of ab125556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.003% EDTA, 0.79% Tris HCl, 0.004% DTT, 0.002% PMSF, 0.31% Glutathione
常规信息
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别名
- KIAA0931
- PH domain and leucine rich repeat protein phosphatase 2
- PH domain leucine rich repeat protein phosphatase like
see all -
功能
Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. -
组织特异性
In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level). -
序列相似性
Contains 22 LRR (leucine-rich) repeats.
Contains 1 PH domain.
Contains 1 PP2C-like domain. -
细胞定位
Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab125556 尚未被引用在任何文献中。