重组人CDC42蛋白(ab87713)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE, WB
描述
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产品名称
重组人CDC42蛋白
参阅全部 CDC42 蛋白酶 -
纯度
> 95 % SDS-PAGE.
ab87713 is purified using conventional chromatography techniques. Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method). -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MASMTGGQQM GRGSHMQTIK CVVVGDGAVG KTCLLISYTT NKFPSEYVPT VFDNYAVTVM IGGEPYTLGL FDTAGQEDYD RLRPLSYPQT DVFLVCFSVV SPSSFENVKE KWVPEITHHC PKTPFLLVGT QIDLRDDPST IEKLAKNKQK PITPETAEKL ARDLKAVKYV ECSALTQKGL KNVFDEAILA ALEPPEPKKS RRC
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab87713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.0154% DTT, 0.316% Tris HCl, 0.0584% EDTA, 10% Glycerol (glycerin, glycerine)
常规信息
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别名
- CDC42
- CDC42_HUMAN
- CDC42Hs
see all -
功能
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia. -
序列相似性
Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily. -
翻译后修饰
AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo. -
细胞定位
Cell membrane. - Information by UniProt
图片
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15% SDS-PAGE showing ab87713 at approximately 22.4kDa (3µg).
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All lanes : Anti-CDC42 antibody (ab64533) at 1 µg/ml
Lane 1 :Recombinant Human CDC42 protein (ab87713) at 0.1 µg
Lane 2 :Recombinant Human CDC42 protein (ab87713) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab87713 尚未被引用在任何文献中。