Application
Western blot
Review text: This CBR4 recombinant protein worked beautifully for our Western blotting positive control purposes.
Sample: Human Recombinant protein (Human rCBR4)
Loading amount: 0.03 µg
Specification: Human rCBR4
Gel Running Conditions: Non-reduced Denaturing (12% Tris/glycine gel)
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Concentration: 1e-005 µg/ml
Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS-Tween 20 (0.05%)
Primary antibody (in addition to 'CBR4 protein')
Primary antibody: Abcam primary antibody: Anti-CBR4 antibody (ab115860)
Dilution: 1/1000
Secondary antibody
Name: Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)
Conjugation: Horse Radish Peroxidase
Dilution: 1/10000
Detection
Detection method: ECL
Exposure: 1 minute(s) and 0 second(s)
Bands: Specific: 32 kDa
Positive control: We used this product as a positive control for western blotting of human CBR4.
Additional data
Additional Notes: Nitrocellulose membranes were used in our WB. The image shows the CBR4 recombinant protein in increasing amounts from left to right and a protein molecular weight ladder on the far right with corresponding molecular weights labeled.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 15 2012