重组人Bmi1蛋白(ab114169)
Key features and details
- Expression system: Wheat germ
- Suitable for: WB, ELISA, SDS-PAGE
描述
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产品名称
重组人Bmi1蛋白
参阅全部 Bmi1 蛋白酶 -
表达系统
Wheat germ -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETS KYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAA HPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKD KEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPL KDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELE SDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSP SGNHQSSFANRPRKASVNGSSATSSG -
预测分子量
62 kDa including tags -
氨基酸
1 to 326
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab114169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
ELISA
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.3% Glutathione, 0.79% Tris HCl
常规信息
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别名
- B lymphoma Mo MLV insertion region (mouse)
- B lymphoma Mo MLV insertion region 1 homolog
- Bmi 1
see all -
功能
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. -
序列相似性
Contains 1 RING-type zinc finger. -
翻译后修饰
Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
图片
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ab114169 on a 12.5% SDS-PAGE Stained with Coomassie Blue.
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Anti-Bmi1 antibody (ab63767) at 2 µg/ml +
Recombinant Human Bmi1 protein (ab114169) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 secondsab63767 recognizes the full length tagged recombinant Bmi1 protein (ab114169) which has an expected molecular weight of 62 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab114169 尚未被引用在任何文献中。