Application
Western blot
Loading amount
1000 µg
Gel Running Conditions
Reduced Denaturing (13)
Sample
Barley (Hordeum vulgare) Tissue lysate - other (embryos and leaves soluble fraction of thylakoids)
Specification
embryos and leaves soluble fraction of thylakoids
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 1/2000
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
chemiluminescent substrate
Bands
Specific: 50 kDa
Exposure
3 minute(s) and 0 second(s)
Positive control
BL21 E.coli culture protein extraction (37kDa) and E.coli RecA recombinant protein (data not shown)
Additional data
Additional Notes
I only observed a positive band on the soluble fraction of the thylakoid extraction protocol from leaves and embryos.
1. BL21 E. coli 1h. culture protein extraction (5 ´l)
2. proteins in the soluble fraction of thylakoids extraction from leaves (15 ´l at 12.5 ´g/´l total protein)
3. proteins in the soluble fraction of thylakoids extraction from embryos (15 ´l at 25 ´g/´l total protein)
4. MW
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Franco Lencina
Verified customer
提交于 Jul 29 2014